Journal: Microbial Cell Factories
Article Title: A workflow to explore elongase diversity and extend the repertoire of fatty acids produced by Yarrowia lipolytica
doi: 10.1186/s12934-025-02890-y
Figure Lengend Snippet: Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely in 96-well format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)
Article Snippet: Cultures were then inoculated at OD 0.5 into 96-well deepwell plates (AB-0661, ABgene, Thermo Scientific) filled with 500 μL of YT 2 D 5 .
Techniques: Comparison, Expressing, Concentration Assay, Standard Deviation